Atg5 Knockout cell line (4T1)

ENCN
location： Home > Products > KO Cell Lines > Atg5 Knockout cell line (4T1)

Atg5 Knockout cell line (4T1) Order

Atg5 Knockout cell line (4T1)
Catalog#
YKO-M225
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Atg5 Knockout cell line (4T1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Atg5 Knockout cell line (4T1)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Mouse
Gene Name
Atg5
Gene ID
11793
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
Accession
CVCL_D7HZ(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2,Exon 3 (based on transcript Atg5-201)
Validation: PCR+Sanger Sequencing
Atg5 Knockout cell line (4T1) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:4T1		 STR Info (Cell bank)
Cell Line:4T1
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 18.019.018.019.0
4-2 21.321.3
6-7 12.012.0
19-2 13.013.0
1-2 17.017.0
7-1 25.225.2
8-1 13.013.0
1-1 15.016.015.016.0
3-2 14.015.014.015.0
2-1 16.017.016.017.0
15-3 22.322.3
6-4 18.018.0
13-1 16.216.2
11-2 18.020.018.019.020.0
17-2 15.015.0
12-1 16.016.0
5-5 14.014.0
X-1 25.025.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Atg5 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 4T1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Atg5 gene knockout Atg5 Knockout cell line (4T1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Atg5 Knockout cell line (4T1)
Atg5 Knockout cell line (4T1)
Catalog#
YKO-M225
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ATG5 Knockout cell line (Hep G2)

    ATG5 Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: ATG5
    Gene ID: 9474
    Size: 1*10^6
    Catalog#: YKO-HT3707
    YKO-HT3707(Hep G2)
  • ATG5 Knockout cell line (THP-1)

    ATG5 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: ATG5
    Gene ID: 9474
    Size: 1*10^6
    Catalog#: YKO-HT0177
    YKO-HT0177(THP-1)
  • Atg5 Knockout cell line (HT-22)

    Atg5 Knockout cell line (HT-22)
      inquiry   More details

    Organism: Mouse
    Gene Name: Atg5
    Gene ID: 11793
    Size: 1*10^6
    Catalog#: YKO-M197
    YKO-M197(HT-22)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ATG5 Knockout cell line (Hep G2)

    ATG5 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: ATG5
    Gene ID: 9474
    Size: 1*10^6
    Catalog#: YKO-HT3707
      inquiry   More details
  • ATG5 Knockout cell line (THP-1)

    ATG5 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: ATG5
    Gene ID: 9474
    Size: 1*10^6
    Catalog#: YKO-HT0177
      inquiry   More details
  • Atg5 Knockout cell line (HT-22)

    Atg5 Knockout cell line (HT-22)

    Organism: Mouse
    Gene Name: Atg5
    Gene ID: 11793
    Size: 1*10^6
    Catalog#: YKO-M197
      inquiry   More details
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now